Pengaruh Pemberian Topikal Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Terhadap Jumlah Sel Fibroblas Pada Penyembuhan Luka Sayat Pada Kulit Tikus Putih (Rattus norvegicus) strain wistar

Abstract

Background: Binahong (Anredera cordifolia (Ten.) Steenis) is a common plant in Indonesia and contain many secondary metabolites such as flavonoids, tannins, saponins, terpenoids, and alkaloids. These secondary metabolite substances were expected to increase the number of fibroblast cells in the wound healing process. This study aims to demonstrate that topical administration of binahong leaf extract jelly on Wistar rats (Rattus norvegicus) skin cut will increase the number of fibroblasts in these wound healing process.

Methods: This is a laboratoric experimental study with randomize post test only control group design. The samples were 25 male Wistar rats aged 3 months and given a dorsal incision of 2 cm long and 0.5 cm deep. The rats were divided randomly into 5 groups (positive control, negative control, and 3 treatment group) consisting of 5 rats each. Positive control group were topically treated with patent medicine Bioplacenton. Negative control group was not given any treatment. Treatment groups were topically treated with a binahong leaf extract jelly 2.5%, 5%, and 10% respectively. Bioplacenton and binahong leaf extract jelly is given 2 times a day on its wound for 5 days. On the fifth day, all rats wound were scored using Bates-Jensen Wound Assassment Tool and then sacrificed to make histophatology slides on the part of the injured skin. The slides were stained with Van Giesson staining and the fibroblast in the granulation tissue was calculated. The data obtained was analyzed with Oneway ANOVA (p <0.05).

Result: The macroscopic wound scoring and average number of fibroblast cells in each group showed no significant difference (p>0.05). However, in the 2nd treatment groups (binahong leaf extract jelly 5%) showed the highest number of fibroblasts (153.72 (SD 23.39)) than the other groups.

Conclusion: Topical administration of binahong leaf extract (Anredera cordifolia (Ten.) Steenis) did not affect the macroscopic wound scoring but it increase the number of fibroblast cells in healing skin cuts on Wistar rats (Rattus norvegicus) even thought it did not reach the significant level.

Keywords: wound healing, fibroblast, binahong (Anredera cordifolia (Ten.) Steenis)