Hepatoprotector effect of Sesbania grandiflora L flowers extract to Liver Cell Structure Damage of Mice caused by Over Reheated Palm Oil

Abstract

Background:Sesbania grandiflora L flower has high antioxidant activity. This activity is caused by phytochemical ingredients of Sesbania grandiflora L flower, such as vitamin C, flavonoids and tannin. The aims of this research were to know the hepatoprotector effect of Sesbania grandiflora L flowers extract to the liver cell structure damage of mice caused by over reheated palm oil and to find out dose escalation of Sesbania grandiflora L flowers extract that can enhance the effect.

Methods:This research was laboratory experimental study with the post-test only controlled group design. The tested animal used in this experiment were male mice, Swiss webster strain.The research population were 30 male mice divided into 5 groups, each group consisted of 6 mice. The negative control group (KKn) was given distilled water, while the positive control group (KKp) was given over reheated palm oil. The 1st treatment group (KP1), the 2nd treatment group (KP2) and the 3rd treatment group (KP3), all, were given Over Reheated Palm Oil as much as 0.06 ml/ 20 gr BW andSesbania grandiflora L flowers extract with dose 7 mg, 14 mg and 28 mg per 20 gr BW of mice, consecutively. The liver histological images were assessed by the sum of the cell nuclear pyknosis, karyorrhexis, and karyolysis. Data were analyzed by using One-Way ANOVA test and Post Hoc Test (LSD) (?=0.05).

Results:One-Way ANOVA test showed that p value was 0.000 (p < 0.05).The results of the post hoc multiple comparisons test showed the significant differences between group KKn-KKp, KKn-KP1, KKn-KP2, KKn-KP3, KKp-KP1, KKp-KP2, KKp-KP3, KP1-KP2, KP1-KP3, and KP2-KP3

Conclusions:Sesbania grandiflora L flowers extract has a protective effect on liver cell structure damage of mice (Mus musculus) caused by over reheated palm oil and the escalated dose of Sesbania grandiflora L flowers extract can enhance the effect.

Keywords:Histological structure of liver damage, over reheated palm oil, Sesbania grandiflora L.